Functionally comparable but evolutionarily distinct nucleotide-targeting effectors help identify conserved paradigms across diverse immune systems

SUPPLEMENTARY MATERIAL

Gianlucca G. Nicastro, A. Maxwell Burroughs, Lakshminarayan M Iyer, and L Aravind

Computational Biology Branch, Intramural Research Program, National Library of Medicine,National Institutes of Health, Bethesda, MD 20894

1. Download all Supplementary files and data as one PDF file.
   • PDF file
2. Supplementary Figures
   • Supplementary Figure S1 - Permuted GreAB-C structure model
   • Supplementary Figure S2 - Double PIN structure model
   • Supplementary Figure S3 - Phylogenetic tree of the Orc/Cdc6-like proteins
   • Supplementary Figure S4 - Sequence logos of different Calcineurin-like phosphoesterase families
   • Supplementary Figure S5 - Activity site and conserved residues from the Calcineurin-CE domain
   • Supplementary Figure S6 - Activity site and conserved residues from the HD-CE domain
   • Supplementary Figure S7 - Activity site and conserved residues from the PRTase-CE domain
   • Supplementary Figure S8 - AlphaFold confidence score for the models produced on the present work
3. Supplementary Data
   A. Genome contextual data
   - AEP-1D
   - AP-ATPase
   - AP-GTPase
   - Dynamin
   - GreAB-C
   - iHD
   - HSP70
   - HSP90
   - ISOCOT
   - MNS
   - NACHT
   - Nadar
   - ORC-Cdc6(Calcineurin-CE)
   - ORC-Cdc6(HD-CE)
   - ORC-Cdc6 (PRTase-CE)
   - ORC-Cdc6 (TIR)
   - ORC-Cdc6(SIR2)
   - ORC-Cdc6(Kinases)
   - ORC-Cdc6(Others)
   - PUA-like
   - ParB-CE
   - ParB
   - Pepco
   - PolBetaCCAA-like
   - RVT
   - SAMHD1
   - nSTAND4
   - nSTAND5
   - iSTAND3
   - iSirtuin
   - STAS big protein
   - STAS
   - Thymidylate synthase
   - iThymidylate synthase
   B. Multiple sequence alignments
   - Calcineurin-CE
   - HD-CE
   - PRTase-CE
   - PUA-like
   - iSirtuin
   - Calcineurin-CE+iThymidylate-synthase
   - AEP-1D
   - REC+Calcineurin-CE
   - STAS+HisKin
   - STAS+PRTase
   - STAS+HisKin+iPRTase+PRTase
   - Pepco
   - iCalcineurin-CE
   - iHD
   C. Phylogenetic Tree (Newick format)
   - Calcineurin-CE Download, view raw data, image
   - HD-CE Download, view raw data, image
   - PRTase-CE Download , view raw data, image
   - ORC-Cdc6 Download, view raw data, image